cy3 streptavidin incubation Search Results


cy3  (Vector Laboratories)
94
Vector Laboratories cy3
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Cy3+Streptavidin/pmc06326903-164-20-29
Average 94 stars, based on 1 article reviews
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streptavidin cy3  (Cytiva Europe)
93
Cytiva Europe streptavidin cy3
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Streptavidin Cy3, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Cy3-Streptavidin/us11883430-754-10-20
Average 93 stars, based on 1 article reviews
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cy3-conjugated streptavidin  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH cy3-conjugated streptavidin
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3 Conjugated Streptavidin, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/cy3+conjugated+streptavidin/10__1523_slash_jneurosci__1870___09__2009-73-13-15
Average 90 stars, based on 1 article reviews
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biotinylated goat anti rabbit secondary antibody  (Jackson Immuno)
96
Jackson Immuno biotinylated goat anti rabbit secondary antibody
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Biotinylated Goat Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Cy+3+Streptavidin/vogel_kara_rain__2014__novel_therapeutics_and_pathomechanisms_in_human_mendelian_disorders_phenylketonuria_maple_syrup_urine-2063-20-26
Average 96 stars, based on 1 article reviews
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cy3 labeled streptoavidin  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology cy3 labeled streptoavidin
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3 Labeled Streptoavidin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/anti-Cy3+Antibody/10__1161_slash_01__atv__0000204635__75748__0f-57-19-6
Average 94 stars, based on 1 article reviews
cy3 labeled streptoavidin - by Bioz Stars, 2026-10
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streptavidin cy3  (Thermo Fisher)
99
Thermo Fisher streptavidin cy3
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Streptavidin Cy3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Streptavidin/pmc04770866-56-9-10
Average 99 stars, based on 1 article reviews
streptavidin cy3 - by Bioz Stars, 2026-10
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streptavidin cy3  (Vector Laboratories)
99
Vector Laboratories streptavidin cy3
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Streptavidin Cy3, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Streptavidin/pmc09736787-157-111-99
Average 99 stars, based on 1 article reviews
streptavidin cy3 - by Bioz Stars, 2026-10
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cy3 streptavidin  (Danaher Inc)
96
Danaher Inc cy3 streptavidin
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3 Streptavidin, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Cy3-Streptavidin/pmc02728133-44-13-14
Average 96 stars, based on 1 article reviews
cy3 streptavidin - by Bioz Stars, 2026-10
96/100 stars
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cy3 conjugated streptavidin antibody  (Jackson Immuno)
96
Jackson Immuno cy3 conjugated streptavidin antibody
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3 Conjugated Streptavidin Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Streptavidin/pmc02551668-54-19-23
Average 96 stars, based on 1 article reviews
cy3 conjugated streptavidin antibody - by Bioz Stars, 2026-10
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streptavidin biotin complex  (Boster Bio)
93
Boster Bio streptavidin biotin complex
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Streptavidin Biotin Complex, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Rabbit+Anti-Goat+IgG+(H%2BL)+Secondary+Antibody%2C+Cy3+Conjugated/pmc07976378-64-34-37
Average 93 stars, based on 1 article reviews
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streptavidin-cy3  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH streptavidin-cy3
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Streptavidin Cy3, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/streptavidin+cy3/10__1172_slash_jci118598-68-9-10
Average 90 stars, based on 1 article reviews
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cy3 streptavidin  (Rockland Immunochemicals)
93
Rockland Immunochemicals cy3 streptavidin
Binding of <t>6D11/Cy3</t> to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a
Cy3 Streptavidin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy3+streptavidin+incubation/Streptavidin+CY3+conjugated/10__1161_slash_01__cir__0000032900__55215__85-71-37-38
Average 93 stars, based on 1 article reviews
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Image Search Results


Binding of 6D11/Cy3 to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a

Journal: Molecular neurobiology

Article Title: Anti-Prion Protein Antibody 6D11 Restores Cellular Proteostasis of Prion Protein through Disrupting Recycling Propagation of PrP Sc and Targeting PrP Sc for Lysosomal Degradation

doi: 10.1007/s12035-018-1208-4

Figure Lengend Snippet: Binding of 6D11/Cy3 to the plasma membrane and its ensuing internalization is significantly greater in N2A/22L than in N2A line. a Representative confocal microphotographs of live N2A and N2A/22L lines incubated with Cy3 conjugated 6D11 mAb (6D11/Cy3) evidencing avid binding of 6D11 mAb to the plasma membrane followed by its progressive internalization. b Corrected total intracellular fluorescence (CTIF) analysis revealing significantly higher internalization of 6D11/Cy3 in N2A/22L line compared to N2A line. Values are given as mean and SEM from n=15 cells per line and treatment time point. b F(3, 98) = 57.23, p < 0.0001 for interaction between the effects of 6D11/Cy3 mAb treatment duration and cell line type; F(3, 98) = 118.8, p < 0.0001 for the main effect of treatment duration and F(1, 98) = 135.9, p < 0.0001 for the main effect of cell line type (two-way analysis of variance); •p < 0.05, ••p < 0.01, ••••p < 0.0001, and ns-non significant, N2A and N2A/22L at various treatment time points vs. N2A and N2A/22L at 0.5 hr. time point, respectively (Holm-Sidak’s multiple comparisons test). **p < 0.01, ****p < 0.0001, and ns-non-significant, N2A vs. N2A/22L for matching treatment time points (Holm-Sidak’s multiple comparisons test). Scale bar 10 μm in a

Article Snippet: Immunocytochemistry reagents: mouse-on-mouse blocking solution, streptavidin/avidin blocking kit, biotinylated secondary antibodies including goat anti-mouse IgG and goat anti-rabbit IgG, and Cy3 and fluorescein isothiocyanate (FITC)-conjugated streptavidin were obtained from Vector Laboratories (Burlingame, CA).

Techniques: Binding Assay, Incubation, Fluorescence